University of the Algarve, Gambelas Campus
|14:00h – 14:30h
||History of animal prion diseases
|14:30h – 15:00h
||History of human prion diseases
15h00 to 18h00 Parallel workshops
W1. Neuropathology and clinicopathological correlation of human prion diseases and related dementias – Organized by Markus Glatzel
Several dementias are characterized by aggregation of abnormally folded conformers of host-encoded proteins. In prion diseases and related dementias, seeded aggregation can lead to the spread of protein aggregates throughout the brain.
In this workshop, we will focus on neuropathology, selective cellular and regional vulnerability, and clinicopathological correlation not only in Creutzfeldt-Jakob disease but also in Parkinson’s, Alzheimer’s disease and related dementias. Besides lectures by prominent experts in the field such as Zane Jaunmuktane, Gabor Kovacs and Markus Glatzel there will be room for case discussions and hands-on neuropathology training in this exciting field of science.
- 15:00h – 15:05h: Markus Glatzel: Introduction to the concept of this workshop
- 15:05h – 15:20h: Markus Glatzel: Pathological deposition of the prion protein and Creutzfeldt-Jakob disease.
- 15:20h – 15:40h: Jane Jaunmuktane: Pathological deposition of the A-beta, tau and alpha-synuclein in Alzheimer’s and Parkinson’s disease
- 15:40h – 16:00h: Gabor Kovacs: Sequential distribution patterns of alpha-synuclein and tau in other neurodegenerative diseases.
- 16:00h – 16:30h: Break
- 16:30h – 17:45h: All speakers and the audience: Case presentation and discussion with selected cases of CJD, AD, DLB, PSP and MSA.
- 17:45h – 18:00h: Markus Glatzel: Summary and feedback.
W2. Cell and animal models of prion and prion-like diseases – organized by Tiago Outeiro
Several dementias are characterized by the accumulation of abnormally folded conformers of host-encoded proteins. However, the precise molecular underpinnings of neurodegeneration are still unclear. In this context, modeling key disease mechanisms in laboratory models is essential in order to enable the scientific community to test therapeutic strategies.
In this workshop, we will focus on cell and animal models of prion and prion-like diseases. We will have lectures by key experts in the field and time for discussions.
- 15:00h – 15:05h: Tiago Outeiro: Introduction of the workshop.
- 15:05h – 15:35h: Jiayi Li: Cell models for studying prion-like diseases.
- 15:35h – 16:05h: Amanda Woerman: Bridging to the clinic: Mouse models of tau and alpha-synuclein prions
- 16:05h – 16:40h: Walker Jackson: Tools to reveal molecular mechanisms in presymptomatic mouse models of Huntington’s and prion diseases.
- 16:40h – 17:00h: Break
- 17:00h – 17:45h: All speakers and the audience: Discussion.
- 17:45h – 18:00h: Tiago Outeiro: Summary and feedback.
W3. Structural biology techniques for studying prion protein in vitro and in silico –Organized by Tuane Vieira
Prion diseases have long been a subject of scientific intrigue and scrutiny due to their multifaceted and elusive nature. A deep understanding of prion protein structures is imperative to comprehend the complex mechanisms at play. In this workshop, we will explore the cutting-edge techniques employed in vitro and in silico to decipher the intricacies of prion proteins.
- 15:00h – 15:05h: Tuane Vieira: Introduction of the workshop
- 15:05h – 15:35h: Yraima Cordeiro: Spectroscopic techniques to study prion protein misfolding and aggregation.
- 15:35h – 16:05h: Holger Wille: Exploring Prion Protein Structure through High-Resolution Techniques
- 16:05h – 16:40h: Salvador Ventura: Fifteen years developing computational tools to study protein aggregation
- 16:40h – 17:00h: Break
- 17:00h – 17:45h: All speakers and the audience: Discussion
- 17:45h – 18:00h: Tuane Vieira: Summary and feedback.
09:00 Opening Session
Rector of University of the Algarve
Vice-President of Faro City Hall, Dr. Paulo Santos
Dean of the Medical School
President of ABC
Director of ABC-RI
Organizing committee representatives
Session 1. Protein structure, function, conversion, dysfunction
Moderator: Alejandro Ruiz
09:30 – 09:30 Keynote lecture: Szymon Manka, Structural biology of prions in 4D: from ex vivo, via in cellulo, towards in situ
10:00 – 10:15 Runchuan Yan, Flexible N-Terminal Domain of the Prion Protein is Neurotoxic
10:15 – 10:30 Luigi Russo, Structural and mechanistic insights into the pathogenesis of prion diseases
10:30 – 10:45 Ilaria Vanni, Isolation of oligomeric prions from atypical scrapie
10:45 – 11:15 Coffee break + posters
Session 2. From yeast prions to mammalian functional aggregates
Moderator: Heather True
11:15 – 12:00 Keynote lecture: Reed Wickner, Anti-prion systems cull all but one in 5000 prions that arise
12:15 – 12:30 Tayyaba Saleem, Isolation and characterization of stress granules from human brain
12:30 – 12:45 Marta Badia, The mutational landscape of the Sup35 QN-rich domain reveals an essential region for Sup35 nucleation
12:45 – 13:00 Yury Chernoff, Relationship between hyperosmotic stress, liquid-liquid phase separation and prion formation
13:00 – 14:30 Lunch break + posters
Session 3. Spreading of pathology in prion-like diseases
Moderator: Amanda Woerman
14:30 – 15:00 Keynote lecture: Mathias Jucker, A-beta seeds
15:00 – 15: 15 Ina Vorberg, Propagation of yeast prions in a mammalian host
15:15 – 15:30 Shelley Forrest, MAPT and SNCA gene expression in cells with protein cytopathologies in Tau- and Synuclein- related neurodegenerative diseases
15:30 – 15:45 Farjana Parvin, Recombinant Aβ amyloid fibrils seed CAA pathology in APP23 transgenic mice
15:45 – 16:45 Coffee break + posters
Session 4. Pathogenic mechanisms in prion diseases
Moderator: Eduardo Melo
16:45 – 17:15 Keynote lecture: David Westaway, Beta-endoproteolysis of the cellular prion protein by dipeptidyl peptidase-4 and fibroblast activation protein
17:15 – 17:30 Giuseppe Legname, A novel prion clearance mechanism by SERPINA3/SerpinA3n
17:30 – 17:45 Prerna Grover, The role of the soluble N-terminal domain (N1-PrP) of the prion protein in αSyn Aggregation and Seeding
17:45 – 18:00 Diane Ritchie, Investigating infectivity associated with prion protein deposits detected in formalin-fixed paraffin- embedded specimens from asymptomatic carriers (UK Appendix studies): an initial update
18:30 Art of Science Event and Welcome Reception
Session 5. The connection between prion diseases and other disorders
Moderator: Tuane Vieira
09:00 – 09:30 Keynote lecture 1: Jerson Silva, Prion-like Aggregation and Phase Transition of Mutant p53 as a Novel Cancer Treatment Strategy
09:30 – 10:00 Keynote lecture 2: Sophie Mouillet-Richard, The prion protein from neurodegeneration to cancer
10:00 – 10:15 Elizabeth Hill, Assessing the Role of Syntaxin-6 on Prion Initiation, Prion Propagation and Prion-Induced Neurotoxicity in vivo
10:15 – 10:30 Holger Wille, A unified approach for rationally designed vaccines targeting neurodegenerative diseases
10:30 – 10:45 Carmen Nussbaum-Krammer, Cellular disaggregation and degradation systems generate seeding and spreading-competent α-Syn and Tau species
10:45 – 11:15 Coffee break + posters
Session 6. Cell biology of PrP
Moderator: Jiyan Ma
11:15 – 11:45 Keynote lecture 1: Christina Sigurdson, Deranged synaptic signaling in experimental prion disease
11:45 – 12:15 Keynote lecture 2: Chiara Zurzolo, Mechanisms of spreading of prions: role of tunneling nanotubes
12:15 – 12:30 Nadia A. Mirza, Murine astrocytes display differential susceptibility to prion strains
12:30 – 12:45 Patricia Aguilar-Calvo, Reducing the sulfation of neuron-derived heparan sulfate accelerates prion protein clearance and prolongs survival time in prion-infected mice
12:45 – 13:00 Walker Jackson, Expression of Rps24-PKE is induced by activated microglia during acquired and genetic prion diseases
13:00 – 14:30 Lunch break + posters
Session 7. Animal prion diseases
Moderator: Leonor Orge
14:30 – 15:00 Keynote lecture: Glenn Telling, Refined transgenetic approaches to characterize the host-range and adaptive potentials of emerging and established chronic wasting disease strains
15:00 – 15:15 Audrey Sandoval, Chronic wasting disease in utero transmission in free-ranging white-tailed deer
15:15 – 15:30 Sabine Gilch, Norwegian moose CWD induces clinical disease and spleen-independent neuroinvasion in mice expressing cervid S138N prion protein
15:30 – 15:45 Francisca Bravo Risi, Detection of CWD prions in plants collected from white-tailed deer farms
15:45 – 16:30 Coffee break + posters
Session 8. Transmission of prions and prion-like proteins
Moderator Tiago Outeiro
16:30 – 17:00 Keynote lecture: Jiyan Ma, Understanding the seeding capability, transmissibility, and pathogenicity of prion
17:00 – 17:15 Sarah A. M. Holec, Effect of neuroinvasion on strain property maintenance for two a-synuclein prion strains
17:15 – 17:30 Joel Watts, Stochastic misfolding drives the emergence of distinct a-synuclein strains
17:30 – 17:45 Rodrigo Morales, Deciphering the pathological significance of self-propagating Aβ strains in different animal models
18:00 – 19:00 Special lecture – John Collinge, A brief history of prions
Session 9. Biomarkers for prion and neurodegenerative diseases
Moderator: Matthias Schmitz
09:00 – 09:30 Keynote lecture: Inga Zerr, Biomarkers for prion and neurodegenerative diseases
09:30 – 09:45 Wen-Quan Zou, Large-Scale Validation of Skin Prion Seed-Amplification Assay for Diagnosis of Creutzfeldt- Jakob Disease
09:45 – 10:00 Michael Geschwind, General and biomarker cerebrospinal fluid findings in prion disease and other rapidly progressive dementias
10:00 – 10:15 Sang-Hyun Oh, Blood-based nano-QuIC: Accelerated and Inhibitor-resistant Detection of Misfolded a-synuclein
10:15 – 11:00 Coffee break + posters
Session 10. CJD International Support Alliance
11:00 – 12:30 Supporting patient associations globally and providing a unified, global voice for all affected by prion disease
12:30 – 14:00 Lunch break + posters
Session 11. Current therapeutic approaches in neurodegeneration
Moderator: Inga Zerr
14:00 – 14:30 Keynote lecture: Jeffrey Kordower, Current therapeutic approaches in neurodegeneration
14:30 – 15:00 Sonia Vallabh, Broad Institute, Lowering PrP: Evidence of therapeutic benefit across diverse yet relevant models
15:00 – 15:30 Pekka Kallunki, Lundbeck, Inhibition of prion like seeding of pathology in Parkinson’s disease by antibodies and other therapies to alpha-synuclein in clinical development
15:30 – 16:00 Warren Hirst, Biogen, Therapeutic approaches for AD
16:00 – 16:45 Coffee break + posters
Session 12. Late breaking news
Moderator: Glenn Telling
16:45 – 17:00 Joaquín Castilla, A Noah’s ark approach to understand the molecular basis of the bona fide spontaneous prion misfolding
17:00 – 17:15 Marcela Viviana Karpuj, A Novel Therapeutic Approach Modulates Protein Interactions and Cellular Processes
17:15 – 17:30 Vineet Rathod, Engineering of a single chained fluobody into a flashbody format for optimized detection and labeling of native PrPSc
17:30 – 17:45 Joseph DeFranco, Peripherally-challenged gene-targeted mice produce strains that recapitulate the properties of natural CWD and are distinct from intracerebrally-adapted variants
18:00 Closing session
19:00 Gala dinner
Departure and Social activities in the region for those interested